LOCUS       sequence-75624-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged human ELL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-F-ELL2
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(285..296)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcatg ccagactaca aggacgatga tgacaagctc
      121 gacatggcgg cgggggggac agggggcctg cgggaggagc agcgctatgg gctgtcgtgc
      181 ggacggctgg ggcaggacaa catcaccgta ctgcatgtga agctcaccga gacggcgatc
      241 cgggcgctcg agacttacca gagccacaag aatttaattc cttttcgacc ttcaatccag
      301 ttccaaggac tccacgggct tgtcaaaatt cccaaaaatg atcccctcaa tgaagttcat
      361 aactttaact tttatttgtc aaatgtgggc aaagacaacc ctcagggcag ctttgactgc
      421 atccagcaaa cattctccag ctctggagcc tcccagctca attgcctggg gtttatacaa
      481 gataaaatta cagtgtgtgc aacaaacgac tcgtatcaga tgacacgaga aagaatgacc
      541 caggcagagg aggaatcccg caaccgaagc acaaaagtta tcaaacccgg tggaccatat
      601 gtagggaaaa gagtgcaaat tcggaaagca cctcaagctg tttcagatac agttcctgag
      661 aggaaaaggt caacccccat gaaccctgca aatacaattc gaaagacaca tagcagcagc
      721 accatctctc agaggccata cagggacagg gtgattcact tactggccct gaaggcctac
      781 aagaaaccgg agctacttgc tagactccag aaagatggtg tcaatcaaaa agacaagaac
      841 tccctgggag caattctgca acaggtagcc aatctgaatt ctaaggacct ctcatatacc
      901 ttaaaggatt atgtttttaa agagcttcaa aga
//
