LOCUS       sequence-75625-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of luciferase driven by mPer2 promoter fragment 
            containing E-box2 (bases -112 to +98 with respect to transcription 
            start site at +1) and SV40 promoter (backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Yoo SH, Ko CH, Lowrey PL, Buhr ED, Song EJ, Chang S, Yoo OJ, 
            Yamazaki S, Lee C, Takahashi JS
  TITLE     A noncanonical E-box enhancer drives mouse Period2 circadian 
            oscillations in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2608-13. Epub 2005 Feb 
            7.
  PUBMED    15699353
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2005-02-15"; volume: "102(7)"; pages: 
            "2608-13. Epub 2005 Feb 7"
COMMENT     Sequence Label: pGL3 Pro E2
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        291..487
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      338..473
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      517..526
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(589..607)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttctctatc gataggtacc gagctcttac gcgtgctagc cccgcggatc cgagcgtagc
       61 tctcaggttc cgccccgcca gtatgcaaat gaggtggcac tccgaccaat ggcgcgcgca
      121 ggggcgggct cagcgcgcgc ggtcacgttt tccactatgt gacagcggag ggcgacgcgg
      181 cggcagcggc gctactggga ctagcggctc cgggcggctg cggcgcaggc cgagcgcacc
      241 aagtgacggg ccgagcaagg gatctagagc agggctcgag atctgcgatc tgcatctcaa
      301 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
      361 ttccgcccat tctccgcccc atcgctgact aatttttttt atttatgcag aggccgaggc
      421 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
      481 ttgcaaaaag cttggcattc cggtactgtt ggtaaagcca ccatggaaga cgccaaaaac
      541 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg
      601 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
      661 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct
      721 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
      781 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
      841 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg
      901 ttcgttt
//
