LOCUS       sequence-75627-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Flag-tagged human AF9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-F-AF9
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcatg ccagactaca aggacgatga tgacaagctc
      121 gagatggcta gctcgtgtgc cgtgcaggtg aagctggagc tggggcaccg cgcccaggtg
      181 aggaaaaaac ccaccgtgga gggcttcacc cacgactgga tggtgttcgt acgcggtccg
      241 gagcacagta acatacagca ctttgtggag aaagtcgtct tccacttgca cgaaagcttt
      301 cctaggccaa aaagagtgtg caaagatcca ccttacaaag tagaagaatc tgggtatgct
      361 ggtttcattt tgccaattga agtttatttt aaaaacaagg aagaacctag gaaagtccgc
      421 tttgattatg acttattcct gcatcttgaa ggccatccac cagtgaatca cctccgctgt
      481 gaaaagctaa ctttcaacaa ccccacagag gactttagga gaaagttgct gaaggcagga
      541 ggggacccta ataggagtat tcataccagc agcagcagca gcagcagcag tagcagcagc
      601 agcagcagca gcagcagcag cagtagcagc agcagcagcg gcagcagcag cagcagtagc
      661 agcagcagta gcagcagcag cagcagcagt agtaccagtt tttcaaagcc tcacaaatta
      721 atgaaggagc acaaggaaaa accttctaaa gactccagag aacataaaag tgccttcaaa
      781 gaaccttcca gggatcacaa caaatcttcc aaagaatcct ctaagaaacc caaagaaaat
      841 aaaccactga aagaagagaa aatagttcct aagatggcct tcaaggaacc taaacccatg
      901 tcaaaagagc caaaaccaga tagtaactta ctcaccatca ccagtgg
//
