LOCUS       sequence-75628-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of luciferase driven by mPer2 promoter fragment 
            containing mutated E-box2 (bases -112 to +98 with respect to 
            transcription start site at +1) and SV40 promoter (backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Yoo SH, Ko CH, Lowrey PL, Buhr ED, Song EJ, Chang S, Yoo OJ, 
            Yamazaki S, Lee C, Takahashi JS
  TITLE     A noncanonical E-box enhancer drives mouse Period2 circadian 
            oscillations in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2608-13. Epub 2005 Feb 
            7.
  PUBMED    15699353
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2005-02-15"; volume: "102(7)"; pages: 
            "2608-13. Epub 2005 Feb 7"
COMMENT     Sequence Label: pGL3 Pro Mut E2
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        289..485
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      336..471
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      515..524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(587..605)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tctctatcga taggtaccga gctcttacgc gtgctagccc cgcggatccg agcgtagctc
       61 tcaggttccg ccccgccagt atgcaaatga ggtggcactc cgaccaatgg cgcgcgcagg
      121 ggcgggctca gcgcgcgcgg tgctagtttc cactatgtga cagcggaggg cgacgcggcg
      181 gcagcggcgc tactgggact agcggctccg ggcggctgcg gcgcaggccg agcgcaccaa
      241 gtgacgggcc gagcaaggga tctagagcag ggctcgagat ctgcgatctg catctcaatt
      301 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      361 ccgcccattc tccgccccat cgctgactaa ttttttttat ttatgcagag gccgaggccg
      421 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      481 gcaaaaagct tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat
      541 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca
      601 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
      661 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat
      721 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
      781 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
      841 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt
      901 cgtttccaaa aaggg
//
