LOCUS       sequence-75629-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression vector. Contains an altered Kozak sequence 
            and/or upstream open reading frames to modulate expression at the 
            level of translation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Ferreira JP, Overton KW, Wang CL
  TITLE     Tuning gene expression with synthetic upstream open reading frames.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 
            10.1073/pnas.1305590110. Epub 2013 Jun 24.
  PUBMED    23798422
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1305590110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-9- 9"; volume: "110"; issue: "28"; pages: "11284-9"
COMMENT     Sequence Label: pCru5-ACC/ACC/ACC-mEGFP-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             80..790
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(119..140)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(380..399)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     727..748
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcctctagac tgccggatct cgagtaacta actaaaccat gggttgataa accatgggtt
       61 gattaccatg ggcgaattca gcaagggcga ggagctgttc accggggtgg tgcccatcct
      121 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      181 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      241 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
      301 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      361 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      421 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      481 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      541 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      601 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      661 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
      721 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      781 gctgtacaag ttcgaataag catgcatcta
//
