LOCUS       sequence-75641-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector. Contains an altered Kozak sequence 
            and/or upstream open reading frames to modulate expression at the 
            level of translation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Ferreira JP, Overton KW, Wang CL
  TITLE     Tuning gene expression with synthetic upstream open reading frames.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 
            10.1073/pnas.1305590110. Epub 2013 Jun 24.
  PUBMED    23798422
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1305590110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-9- 9"; volume: "110"; issue: "28"; pages: "11284-9"
COMMENT     Sequence Label: pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             176..688
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIE"
     primer_bind     complement(215..236)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(476..495)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     823..844
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
       61 gggtttgccg ccagaacaca ggtgtcgtga cgcgcatcca ggcctaagct tacgctcgag
      121 taactaacta aaccatgggt tgataaacca tgggttgatt accatgggcg aattcagcaa
      181 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      241 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      301 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      361 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      421 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      481 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      541 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      601 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      661 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      721 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      781 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      841 cgtgaccgcc gccgggatca ctctcggcat ggacga
//
