LOCUS       sequence-75661-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-TarA
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(128..155)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(171..242)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             935..946
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    964..983
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtagc
       61 tagaacatac cacacggtat gtttagctgt caattggtaa cgaatcggat gtattagagg
      121 ctataaacgc agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc
      181 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
      241 tgatgcctgg gtccacttgt atgttttaac atgcaatacc gctcagatca ccgctacgac
      301 gacgtgcttc tgcaataaca cgtgcaccac gatccggtgc ggtatccaga cgggtcagaa
      361 caggcggtgc tgcaattgca cccagcagat ggctatacag tgctgcggta cgatcatcca
      421 gatcttccca acctgccagg gtctggctaa caaactgtgc accaacccat gcacccagaa
      481 aaacctgtgc ggtttcttcc ggaacaacat gcggcagaac ttcaccacgt ttttttgctt
      541 cggtcagaac atggctggtc agacgtgacc atgcaggcca tgcgctaccc agaacttcac
      601 gacccggacg atctgcgcta atacgaacgc ctgccagcag aaacggttca cgtggcagac
      661 gacgtgccag ggtcatacct gcatcaaccc attcttgcag tttcagttca cgaggcagat
      721 gatattccgg tgtcagctgt tcttgcagaa caccctgtgc cagtgctgct ttgctatcaa
      781 agtgaaaata cagtgcacct ttggtaacac cggcacgttt cagaatttcg ctaatggttg
      841 ctgctgcata accacgttct gcaaaaactg ctgctgctgc acgcagaact gcacgacggg
      901 tacgaactgc acgatcctgc tgtgccatct agtatttctc ctctttgaaa acatagtcca
      961 tagaattgtg agcgctcaca attccacaca ttatacgagc
//
