LOCUS       sequence-75667-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-TetR
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(23..736,737..739,740..742))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKLHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(68..89)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     417..436
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     676..697
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     RBS             749..760
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    804..822
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(844..871)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tccgccaaaa cagccaagct tattacttgt acagctcgtc catgccgaga gtgatcccgg
       61 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
      121 gggcggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgattg
      181 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
      241 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
      301 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      361 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      421 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
      481 cggacttgaa gaagtcgtgc agcttcatgt ggtcggggta gcgggcgaag cattgcaggc
      541 cgtagccgaa ggtggtcacg agggtgggcc agggcacggg cagcttgcct gtggtgcaga
      601 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
      661 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
      721 gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtagt gctcattata
      781 tctatcactg atagggatgt caatctctat cactgatagg gattccaccg tacgtcgtat
      841 aaacgcagaa aggcccaccc gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg
      901 acaaacaaca ga
//
