LOCUS       sequence-75698-e        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses an attenuated T7 RNAP under an isopropyl 
            beta-d-1-thiogalactopyranoside (IPTG) inducible control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pN565
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(368..397)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 agaagaagat cgcttggcct cgcgcacaga tcagttggaa gaatttgtcc actacgtgaa
       61 aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg
      121 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca
      181 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
      241 tggtaccctc cggcaaaaaa acgggcaagg tgtcaccacc ctgccctttt tctttaaaac
      301 cgaaaagatt acttcgcgtt tgccacctga cgtctaagaa aaggaatatt cagcaatttg
      361 cccgtgccga agaaaggccc acccgtgaag gtgagccagt gagttgattg ctacgtaatt
      421 agttagttag cccttagtga ctcgaattct tggcagcttc tagagcaatt cgcgctaact
      481 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      541 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg
      601 tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag
      661 agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg
      721 tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga
      781 tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct
      841 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt
      901 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat
      961 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg
     1021 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
//
