LOCUS       sequence-75704-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mutant version of the wild-type miR-16-1 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir16-1.1
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(240..264)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             617..658
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(698..717)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    742..790
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gaaattaata cgactcacta tagggagacc caagctggct agttaagcta tcaacaagtt
       61 tgtacaaaaa agcaggctat atttcttttt attcatagct cttatgatag caaactcagc
      121 agtgccttag cagcacgtaa atattggcgt taagattcta aaattatctc cagtattaac
      181 tgtgctgctg aagtaaggtt gagcatactc tacagttgtg ttttaatgta tattaatgtt
      241 cccagctttc ttgtacaaag tggttgatct agagggcccg cagtggggtc agcttctacc
      301 ggggcggcgt cccggggtct tggaactgca tgcagactgc ctgcttggga aacatacttc
      361 tttatatgcc catatggacc tgctaagcta tggaatgtaa agaagtatgt atctcaggcc
      421 gggacctctc tcgccgcact gaggggcact ccacaccacg ggggccgccg cggccgacag
      481 ccagccaggc ggctgtggtg ggggtgaggc tggggcggca ggctgcctgg gcgttggtgg
      541 acggtcgtgt ccccgccagc attgttctgt cactttgcct gtgtcagcag gtggaagtgc
      601 tcaccagaca ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
      661 taccggttag taatgagttt aaacggggga ggctaactga aacacggaag gagacaatac
      721 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg
      781 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
      841 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca cccccc
//
