LOCUS       sequence-75707-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mutant version of the wild-type miR-16-1 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir16-1.2
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(239..263)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             616..657
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(697..716)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    741..789
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 aaattaatac gactcactat agggagaccc aagctggcta gttaagctat caacaagttt
       61 gtacaaaaaa gcaggctata tttcttttta ttcatagctc ttatgatagc aatgtcagca
      121 gtgccttagc agcacgtaaa tattggcgtt aagattctaa aattatctcc agtattaact
      181 gtgctgctga agtaaggttg accatacttt atagttgtgt tttaatgtat attaatgttc
      241 ccagctttct tgtacaaagt ggttgatcta gagggcccgc agtggggtca gcttctaccg
      301 gggcggcgtc ccggggtctt ggaactgcat gcagactgcc tgcttgggaa acatacttct
      361 ttatatgccc atatggacct gctaagctat ggaatgtaaa gaagtatgta tctcaggccg
      421 ggacctctct cgccgcactg aggggcactc cacaccacgg gggccgccgc ggccgacagc
      481 cagccaggcg gctgtggtgg gggtgaggct ggggcggcag gctgcctggg cgttggtgga
      541 cggtcgtgtc cccgccagca ttgttctgtc actttgcctg tgtcagcagg tggaagtgct
      601 caccagacat tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
      661 accggttagt aatgagttta aacgggggag gctaactgaa acacggaagg agacaatacc
      721 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
      781 cgtttgttca taaacgcggg
//
