LOCUS       sequence-75711-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mutant version of the wild-type miR-16-1 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir16-1.3
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(241..265)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             618..659
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(699..718)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    743..791
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 cgaaattaat acgactcact atagggagac ccaagctggc tagttaagct atcaacaagt
       61 ttgtacaaaa aagcaggcta tatttctttt tattcatagc tcttatgata gcaaactcag
      121 cagtgcctta gcagcacgta aatattggcg ttaagattct aaaattatct ccagtattaa
      181 ctgtgctgct gaagtaaggt tgagcatact ttatagttgt gttttaatgt atattaatgt
      241 tcccagcttt cttgtacaaa gtggttgatc tagagggccc gcagtggggt cagcttctac
      301 cggggcggcg tcccggggtc ttggaactgc atgcagactg cctgcttggg aaacatactt
      361 ctttatatgc ccatatggac ctgctaagct atggaatgta aagaagtatg tatctcaggc
      421 cgggacctct ctcgccgcac tgaggggcac tccacaccac gggggccgcc gcggccgaca
      481 gccagccagg cggctgtggt gggggtgagg ctggggcggc aggctgcctg ggcgttggtg
      541 gacggtcgtg tccccgccag cattgttctg tcactttgcc tgtgtcagca ggtggaagtg
      601 ctcaccagac attcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
      661 gtaccggtta gtaatgagtt taaacggggg aggctaactg aaacacggaa ggagacaata
      721 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
      781 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
      841 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc acccccc
//
