LOCUS       sequence-75718-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Left TALEN for PRKAG2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga 
            PP, So PL, Conklin BR
  TITLE     Isolation of single-base genome-edited human iPS cells without 
            antibiotic selection.
  JOURNAL   Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 
            2014 Feb 9.
  PUBMED    24509632
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2840"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "291-3"
COMMENT     Sequence Label: MR015-PRKAG2 Left TALEN
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      87..96
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..161
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             168..188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgcca ccatggacta caaagaccat gacggtgatt
      121 ataaagatca tgacatcgat tacaaggatg acgatgacaa gatggccccc aagaagaaga
      181 ggaaggtggg cattcaccgc ggggtaccta tggtggactt gaggacactc ggttattcgc
      241 aacagcaaca ggagaaaatc aagcctaagg tcaggagcac cgtcgcgcaa caccacgagg
      301 cgcttgtggg gcatggcttc actcatgcgc atattgtcgc gctttcacag caccctgcgg
      361 cgcttgggac ggtggctgtc aaataccaag atatgattgc ggccctgccc gaagccacgc
      421 acgaggcaat tgtaggggtc ggtaaacagt ggtcgggagc gcgagcactt gaggcgctgc
      481 tgactgtggc gggtgagctt agggggcctc cgctccagct cgacaccggg cagctgctga
      541 agatcgcgaa gagaggggga gtaacagcgg tagaggcagt gcacgcctgg cgcaatgcgc
      601 tcaccggtgc ccccctgaac ctgaccccgg accaagtggt ggctatcgcc agccacgatg
      661 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
      721 gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg
      781 aaacggtgca gcggctgttg ccggtgctgt gccagga
//
