LOCUS       sequence-75719-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Right TALEN for PRKAG2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga 
            PP, So PL, Conklin BR
  TITLE     Isolation of single-base genome-edited human iPS cells without 
            antibiotic selection.
  JOURNAL   Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 
            2014 Feb 9.
  PUBMED    24509632
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2840"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "291-3"
COMMENT     Sequence Label: MR015-PRKAG2 Right TALEN
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..162
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             169..189
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgcc accatggact acaaagacca tgacggtgat
      121 tataaagatc atgacatcga ttacaaggat gacgatgaca agatggcccc caagaagaag
      181 aggaaggtgg gcattcaccg cggggtacct atggtggact tgaggacact cggttattcg
      241 caacagcaac aggagaaaat caagcctaag gtcaggagca ccgtcgcgca acaccacgag
      301 gcgcttgtgg ggcatggctt cactcatgcg catattgtcg cgctttcaca gcaccctgcg
      361 gcgcttggga cggtggctgt caaataccaa gatatgattg cggccctgcc cgaagccacg
      421 cacgaggcaa ttgtaggggt cggtaaacag tggtcgggag cgcgagcact tgaggcgctg
      481 ctgactgtgg cgggtgagct tagggggcct ccgctccagc tcgacaccgg gcagctgctg
      541 aagatcgcga agagaggggg agtaacagcg gtagaggcag tgcacgcctg gcgcaatgcg
      601 ctcaccggtg cccccctgaa cctgaccccg gaccaagtgg tggctatcgc cagcaacatt
      661 ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat
      721 ggcctgactc cggaccaagt ggtggctatc gccagccacg atggcggcaa gcaagcgctc
      781 gaaacggtgc agcggctgtt gccggtgctg tgcca
//
