LOCUS       sequence-75720-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Left TALEN for PKP2 with NK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga 
            PP, So PL, Conklin BR
  TITLE     Isolation of single-base genome-edited human iPS cells without 
            antibiotic selection.
  JOURNAL   Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 
            2014 Feb 9.
  PUBMED    24509632
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2840"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "291-3"
COMMENT     Sequence Label: MR015-PKP2 Left TALEN_NK
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..159
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             166..186
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgccacc atggactaca aagaccatga cggtgattat
      121 aaagatcatg acatcgatta caaggatgac gatgacaaga tggcccccaa gaagaagagg
      181 aaggtgggca ttcaccgcgg ggtacctatg gtggacttga ggacactcgg ttattcgcaa
      241 cagcaacagg agaaaatcaa gcctaaggtc aggagcaccg tcgcgcaaca ccacgaggcg
      301 cttgtggggc atggcttcac tcatgcgcat attgtcgcgc tttcacagca ccctgcggcg
      361 cttgggacgg tggctgtcaa ataccaagat atgattgcgg ccctgcccga agccacgcac
      421 gaggcaattg taggggtcgg taaacagtgg tcgggagcgc gagcacttga ggcgctgctg
      481 actgtggcgg gtgagcttag ggggcctccg ctccagctcg acaccgggca gctgctgaag
      541 atcgcgaaga gagggggagt aacagcggta gaggcagtgc acgcctggcg caatgcgctc
      601 accggtgccc ccctgaacct gaccccggac caagtggtgg ctatcgccag caacaagggc
      661 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
      721 ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctcgaa
      781 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc
//
