LOCUS       sequence-75722-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild-type miR-16-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir16-1 wt
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8..26
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(242..266)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             619..660
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(700..719)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    744..792
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagttaagc tatcaacaag
       61 tttgtacaaa aaagcaggct atatttcttt ttattcatag ctcttatgat agcaatgtca
      121 gcagtgcctt agcagcacgt aaatattggc gttaagattc taaaattatc tccagtatta
      181 actgtgctgc tgaagtaagg ttgaccatac tctacagttg tgttttaatg tatattaatg
      241 ttcccagctt tcttgtacaa agtggttgat ctagagggcc cgcagtgggg tcagcttcta
      301 ccggggcggc gtcccggggt cttggaactg catgcagact gcctgcttgg gaaacatact
      361 tctttatatg cccatatgga cctgctaagc tatggaatgt aaagaagtat gtatctcagg
      421 ccgggacctc tctcgccgca ctgaggggca ctccacacca cgggggccgc cgcggccgac
      481 agccagccag gcggctgtgg tgggggtgag gctggggcgg caggctgcct gggcgttggt
      541 ggacggtcgt gtccccgcca gcattgttct gtcactttgc ctgtgtcagc aggtggaagt
      601 gctcaccaga cattcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
      661 cgtaccggtt agtaatgagt ttaaacgggg gaggctaact gaaacacgga aggagacaat
      721 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
      781 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
      841 cgagacccca ttggggccaa tacgcccgcg ttttc
//
