LOCUS       sequence-75728-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir28-1
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    96..120
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(311..335)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             688..729
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(769..788)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    813..861
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagttaagc tatcaacaag tttgtacaaa aaagcaggct
      121 ggtagacaac atgcttgatg ctgaagatac aagaaaaaat ttaaaacagt ccttgccctc
      181 aaggagctca cagtctattg agttaccttt ctgactttcc cactagattg tgagctcctg
      241 gagggcaggc actttcgttc atctgaaaaa gagcttaaat ttcagtgtta atcctagatt
      301 acaatcccgc ccccagcttt cttgtacaaa gtggttgatc tagagggccc gcagtggggt
      361 cagcttctac cggggcggcg tcccggggtc ttggaactgc atgcagactg cctgcttggg
      421 aaacatactt ctttatatgc ccatatggac ctgctaagct atggaatgta aagaagtatg
      481 tatctcaggc cgggacctct ctcgccgcac tgaggggcac tccacaccac gggggccgcc
      541 gcggccgaca gccagccagg cggctgtggt gggggtgagg ctggggcggc aggctgcctg
      601 ggcgttggtg gacggtcgtg tccccgccag cattgttctg tcactttgcc tgtgtcagca
      661 ggtggaagtg ctcaccagac attcgaaggt aagcctatcc ctaaccctct cctcggtctc
      721 gattctacgc gtaccggtta gtaatgagtt taaacggggg aggctaactg aaacacggaa
      781 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
      841 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
      901 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
      961 accccc
//
