LOCUS       sequence-75736-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir28-2
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8..26
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(271..295)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             648..689
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(729..748)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagttaagc tatcaacaag
       61 tttgtacaaa aaagcaggct ggtagacaac atgcttgatg ctgaagatac aagaaaaaat
      121 ttaaaatggt ccttgccctc aaggagctca cagtctattg agttaccttt ctgactttcc
      181 cactagattg tgagctcctg gagggcaggc actttcgtta atatgaaaaa gagcttaaat
      241 ttcagtgtta atcctagatt acaatcccgc ccccagcttt cttgtacaaa gtggttgatc
      301 tagagggccc gcagtggggt cagcttctac cggggcggcg tcccggggtc ttggaactgc
      361 atgcagactg cctgcttggg aaacatactt ctttatatgc ccatatggac ctgctaagct
      421 atggaatgta aagaagtatg tatctcaggc cgggacctct ctcgccgcac tgaggggcac
      481 tccacaccac gggggccgcc gcggccgaca gccagccagg cggctgtggt gggggtgagg
      541 ctggggcggc aggctgcctg ggcgttggtg gacggtcgtg tccccgccag cattgttctg
      601 tcactttgcc tgtgtcagca ggtggaagtg ctcaccagac attcgaaggt aagcctatcc
      661 ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagtt taaacggggg
      721 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
      781 aaaagacaga ataaaacgca cgggtgttgg g
//
