LOCUS       sequence-75737-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Right TALEN for PHOX2B with NN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga 
            PP, So PL, Conklin BR
  TITLE     Isolation of single-base genome-edited human iPS cells without 
            antibiotic selection.
  JOURNAL   Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 
            2014 Feb 9.
  PUBMED    24509632
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2840"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "291-3"
COMMENT     Sequence Label: MR015-PHOX2BRightTALEN_NN
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             92..157
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             164..184
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagctggcta gcgccaccat ggactacaaa gaccatgacg gtgattataa
      121 agatcatgac atcgattaca aggatgacga tgacaagatg gcccccaaga agaagaggaa
      181 ggtgggcatt caccgcgggg tacctatggt ggacttgagg acactcggtt attcgcaaca
      241 gcaacaggag aaaatcaagc ctaaggtcag gagcaccgtc gcgcaacacc acgaggcgct
      301 tgtggggcat ggcttcactc atgcgcatat tgtcgcgctt tcacagcacc ctgcggcgct
      361 tgggacggtg gctgtcaaat accaagatat gattgcggcc ctgcccgaag ccacgcacga
      421 ggcaattgta ggggtcggta aacagtggtc gggagcgcga gcacttgagg cgctgctgac
      481 tgtggcgggt gagcttaggg ggcctccgct ccagctcgac accgggcagc tgctgaagat
      541 cgcgaagaga gggggagtaa cagcggtaga ggcagtgcac gcctggcgca atgcgctcac
      601 cggtgccccc ctgaacctga ccccggacca agtggtggct atcgccagcc acgatggcgg
      661 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg acc
//
