LOCUS       sequence-75738-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir28-3
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(316..340)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             693..734
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(774..793)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    818..866
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt acaaaaaagc
      121 aggctggtag acaacatgct tgatgctgaa gatacaagaa aaaatttaaa acagtccttg
      181 ccctcaagga gctcacagtc tattgagtta cctttctgac tttcccacta gattgtgagc
      241 tcctggaggg caggcacttt cgttaatatg aaaaagagct taaatttcag tgttaatcct
      301 agattacaat cccgccccca gctttcttgt acaaagtggt tgatctagag ggcccgcagt
      361 ggggtcagct tctaccgggg cggcgtcccg gggtcttgga actgcatgca gactgcctgc
      421 ttgggaaaca tacttcttta tatgcccata tggacctgct aagctatgga atgtaaagaa
      481 gtatgtatct caggccggga cctctctcgc cgcactgagg ggcactccac accacggggg
      541 ccgccgcggc cgacagccag ccaggcggct gtggtggggg tgaggctggg gcggcaggct
      601 gcctgggcgt tggtggacgg tcgtgtcccc gccagcattg ttctgtcact ttgcctgtgt
      661 cagcaggtgg aagtgctcac cagacattcg aaggtaagcc tatccctaac cctctcctcg
      721 gtctcgattc tacgcgtacc ggttagtaat gagtttaaac gggggaggct aactgaaaca
      781 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
      841 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
      901 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
      961 accccacccc ccaagtt
//
