LOCUS       sequence-75780-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir193b-2
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(282..306)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             659..700
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(740..759)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    784..832
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
       61 tggctagtta agctatcaac aagtttgtac aaaaaagcag gctgccgtga atggggactc
      121 acttcttggg aaaagaggct tttggaggct gtggtctcag aatcggggtt ttgagggcga
      181 gatgagttta tgttttatcc aactggccct caaagtcccg cttttggggt cattctggat
      241 agtgagggac tcaggccccg gcagtctcgg gagggtgagg gccccagctt tcttgtacaa
      301 agtggttgat ctagagggcc cgcagtgggg tcagcttcta ccggggcggc gtcccggggt
      361 cttggaactg catgcagact gcctgcttgg gaaacatact tctttatatg cccatatgga
      421 cctgctaagc tatggaatgt aaagaagtat gtatctcagg ccgggacctc tctcgccgca
      481 ctgaggggca ctccacacca cgggggccgc cgcggccgac agccagccag gcggctgtgg
      541 tgggggtgag gctggggcgg caggctgcct gggcgttggt ggacggtcgt gtccccgcca
      601 gcattgttct gtcactttgc ctgtgtcagc aggtggaagt gctcaccaga cattcgaagg
      661 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgagt
      721 ttaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
      781 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
      841 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttgggg
//
