LOCUS       sequence-75784-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir193b-3
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    90..114
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(293..317)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             670..711
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(751..770)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag ctggctagtt aagctatcaa caagtttgta caaaaaagca ggctgccgtg
      121 aatggggact cacttcttgg gaaaagaggc ttttggaggc catggtctca gaatcggggt
      181 tttgagggcg agatgagttt atgttttatc caactggccc tcaaagtccc gcttttgggg
      241 tcattctgga tagtgaggga ctcaggcccc ggcagtctcg ggagggtgag ggccccagct
      301 ttcttgtaca aagtggttga tctagagggc ccgcagtggg gtcagcttct accggggcgg
      361 cgtcccgggg tcttggaact gcatgcagac tgcctgcttg ggaaacatac ttctttatat
      421 gcccatatgg acctgctaag ctatggaatg taaagaagta tgtatctcag gccgggacct
      481 ctctcgccgc actgaggggc actccacacc acgggggccg ccgcggccga cagccagcca
      541 ggcggctgtg gtgggggtga ggctggggcg gcaggctgcc tgggcgttgg tggacggtcg
      601 tgtccccgcc agcattgttc tgtcactttg cctgtgtcag caggtggaag tgctcaccag
      661 acattcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
      721 tagtaatgag tttaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
      781 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt ggg
//
