LOCUS       sequence-75797-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial T. denticola Cas9 (TD) + tracrRNA expression, 
            cloDF13/spectinomycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-TDcas
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctgtacctgt actacaccca gctgggcaag tgcatgtact gcggcaagcc catcgagatc
       61 ggccacgtgt tcgacaccag caactacgac atcgaccaca tctaccccca gagcaagatc
      121 aaggacgaca gcatcagcaa ccgcgtgctg gtgtgcagca gctgcaacaa gaacaaggag
      181 gacaagtacc ctctgaagag cgagatccag agcaagcagc gcggcttctg gaacttcctg
      241 cagcgcaaca acttcatcag cctggagaag ctgaaccgcc tgacccgcgc cacccccatc
      301 agcgacgacg agaccgccaa gttcatcgcc cgccagctgg tggagactcg ccaagctacc
      361 aaggtggccg ccaaggtgct ggagaagatg ttccccgaga ccaagatcgt gtacagcaag
      421 gccgagaccg tgagcatgtt ccgcaacaag ttcgacatcg tgaagtgccg cgagatcaac
      481 gacttccacc acgcccacga cgcctacctg aacatcgtgg tgggcaacgt gtacaacacc
      541 aagttcacca acaacccctg gaatttcatt aaggagaagc gcgacaaccc caagatcgcc
      601 gacacctaca actactacaa ggtgttcgac tacgacgtga agcgcaacaa catcaccgcc
      661 tgggagaagg gcaagaccat catcaccgtg aaggacatgc tgaagcgcaa cacccccatc
      721 tacacccgcc aggccgcctg caagaagggc gagctgttca accagaccat catgaagaag
      781 ggcctgggcc agcaccccct gaagaaggag ggccccttca gcaacatcag caagtacggc
      841 ggctacaaca aggtgagcgc cgcctactac accctgatcg agtac
//
