LOCUS       sequence-75798-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-tagged ER wild-type in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Toy W, Shen Y, Won H, Green B, Sakr RA, Will M, Li Z, Gala K, 
            Fanning S, King TA, Hudis C, Chen D, Taran T, Hortobagyi G, Greene 
            G, Berger M, Baselga J, Chandarlapaty S
  TITLE     ESR1 ligand-binding domain mutations in hormone-resistant breast 
            cancer.
  JOURNAL   Nat Genet. 2013 Dec;45(12):1439-45. doi: 10.1038/ng.2822. Epub 2013 
            Nov 3.
  PUBMED    24185512
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2822"; 
            journalName: "Nat Genet"; date: "2013-12"; volume: "45"; issue: 
            "12"; pages: "1439-45"
COMMENT     Sequence Label: pcDNA-HA-ER WT
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             123..149
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag ctggctagcg tttaaactta agcttggtac cgagctcgga tccgccacca
      121 tgtatccata tgacgtccca gactatgcca ccatgaccct ccacaccaaa gcatctggga
      181 tggccctact gcatcagatc caagggaacg agctggagcc cctgaaccgt ccgcagctca
      241 agatccccct ggagcggccc ctgggcgagg tgtacctgga cagcagcaag cccgccgtgt
      301 acaactaccc cgagggcgcc gcctacgagt tcaacgccgc ggccgccgcc aacgcgcagg
      361 tctacggtca gaccggcctc ccctacggcc ccgggtctga ggctgcggcg ttcggctcca
      421 acggcctggg gggtttcccc ccactcaaca gcgtgtctcc gagcccgctg atgctactgc
      481 acccgccgcc gcagctgtcg cctttcctgc agccccacgg ccagcaggtg ccctactacc
      541 tggagaacga gcccagcggc tacacggtgc gcgaggccgg cccgccggca ttctacaggc
      601 caaattcaga taatcgacgc cagggtggca gagaaagatt ggccagtacc aatgacaagg
      661 gaagtatggc tatggaatct gccaaggaga ctcgctactg tgcagtgtgc aatgactatg
      721 cttcaggcta ccattatgga gtctggtcct gtgagggctg caaggccttc ttcaagagaa
      781 gtattcaagg acataacgac tatatgtgtc cagccaccaa ccagtgcacc attgataaaa
      841 acaggaggaa gagctgccag gcctgccggc tccgcaaatg ctacgaagtg ggaatgatga
      901 aaggtgggat acgaaaaga
//
