LOCUS       sequence-75800-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependant (flex) rabies glycoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A
  TITLE     Dissecting local circuits: parvalbumin interneurons underlie broad 
            feedback control of olfactory bulb output.
  JOURNAL   Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027.
            Epub 2013 Nov 14.
  PUBMED    24239125
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.08.027"; journalName: "Neuron"; date: 
            "2013-12-4- 4"; volume: "80"; issue: "5"; pages: "1232-45"
COMMENT     Sequence Label: CAG-Flex-RG
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    101..134
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    197..230
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
       61 tttggcaaag aattgatttg ataccgcggg cccggccggg ataacttcgt ataaagtatc
      121 ctatacgaag ttatatcaaa ataggaagac caatgcttca ccatcgaccc gaattgccaa
      181 gcatcaccat cgacacataa cttcgtataa tgtatgctat acgaagttat gtcgacttac
      241 agtctggtct cacccccact cttgtgtgat tcccatgaag atatgatctt cccgctttgg
      301 ggagtgactg acacctccct ccctgtccct ctgagattgt gttgcgtagg ttctgatcga
      361 ttgactcttc tacaacatgt catcaggaaa attatcaaca tcaaggcagt cagggcccct
      421 gcactcagta atacatactt cccccagttc gggagaccca agtcaactcc tgagacctga
      481 ttgtgcacat cgggaaggtg aacttcaaca aaatcctcag cctcgtcacc gtccttgaaa
      541 acggtagacg ggtctgccag ggggtgcaca agggggataa ccgaggattc caacaactcc
      601 atatgttgct ggaggaggga tgattgcatc tctgggatta agacattgcc gtcaggtcct
      661 aatattatac cattgaaaaa caccccgttc acatgaggat gacacctccc cccaactctt
      721 aaacaccctt ttgaagggag gatctcattc caagttctga ctgacttgta gtgagcatcg
      781 gcttccatca aggtcttgtt gaatatggta tatgcttttc c
//
