LOCUS       sequence-75803-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Ernst R, Mueller B, Ploegh HL, Schlieker C
  TITLE     The otubain YOD1 is a deubiquitinating enzyme that associates with 
            p97 to facilitate protein dislocation from the ER.
  JOURNAL   Mol Cell. 2009 Oct 9;36(1):28-38. doi: 10.1016/j.molcel.2009.09.016.
  PUBMED    19818707
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.09"; journalName: "Mol Cell"; date: 
            "2009-10-9- 9"; volume: "36"; issue: "1"; pages: "28-38"
COMMENT     Sequence Label: pcDNA3-Flag-YOD1 C160S
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagcg tttaaactta agcttgccac catggattac aaggatgacg acgataagat
      121 gtttggcccc gctaaaggtc gccattttgg agtccacccg gcgcctggtt tccccggcgg
      181 cgtctcccaa caggctgccg ggaccaaagc tggccccgcg ggtgcctggc ctgtgggcag
      241 ccggaccgac acgatgtggc ggctccgctg caaggccaag gacggcaccc atgttttgca
      301 ggggctgtcc agccggaccc gggtgcggga actccagggc caaattgccg ccatcaccgg
      361 gatcgccccc ggcggtcagc gaatcctcgt cggataccct cccgagtgcc tggatctcag
      421 caatggggat accattctgg aagacttgcc catccaatct ggtgacatgc tgatcattga
      481 agaagaccaa accaggccca gaagttcacc tgcatttact aaacgtggtg cttctagtta
      541 cgtcagggaa actttgcctg tgcttaccag aaccgtggtc ccagcagaca actcttccct
      601 ctttactagt gtgtactatg tcgtcgaagg aggagtcttg aatccagctt gtgcccctga
      661 gatgagacgc ctcatagcac aaattgtagc aagcgatcca gacttctata gtgaggcaat
      721 actgggaaaa acaaatcaag agtactgtga ctggatcaaa agggatgaca cttggggagg
      781 agcaatagag atatcgattt tgtccaagtt ttaccaatgt gaaatatgtg tagtggatac
      841 acagacagta agaattgatc gttttgggga agatgcagga tataccaaaa gggttctgct
      901 tatttatgat ggcatccact atgatccact tcagcgtaac ttccctgatc cagatacacc
//
