LOCUS       sequence-75807-e         374 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB63
FEATURES             Location/Qualifiers
     source          1..374
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(311..331)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(338..361)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aggagtcgtc gaccttggcc atctcgttgg agaagatctc ttggaggtag cagatacggt
       61 tcttacgacg ggtgtaacga cgacgggcgg tacgcttgag acgggtggcc tcggcggtct
      121 ctccggagtc gaagaggagg gctccgatga ggttcttctt gatggagtga cggtcggtgt
      181 ttccgaggac cttgaacttc ttggatggga ccttgtactc gtcggtgatg acggcccatc
      241 cgacggagtt ggttccgatg tcgagtccga tggagtactt cttgtcggcg gctgggactc
      301 cgtggattcc gaccttacgc ttcttctttg gggccatctt gtcgtcgtcg tccttgtagt
      361 cgatgtcgtg tctt
//
