LOCUS       sequence-75808-e         525 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB66
FEATURES             Location/Qualifiers
     source          1..525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(312..332)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(339..404)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gaggagtcgt cgaccttggc catctcgttg gagaagatct cttggaggta gcagatacgg
       61 ttcttacgac gggtgtaacg acgacgggcg gtacgcttga gacgggtggc ctcggcggtc
      121 tctccggagt cgaagaggag ggctccgatg aggttcttct tgatggagtg acggtcggtg
      181 tttccgagga ccttgaactt cttggatggg accttgtact cgtcggtgat gacggcccat
      241 ccgacggagt tggttccgat gtcgagtccg atggagtact tcttgtcggc ggctgggact
      301 ccgtggattc cgaccttacg cttcttcttt ggggccatct tgtcgtcgtc gtccttgtag
      361 tcgatgtcgt ggtccttgta gtctccgtcg tggtccttgt agtccatttt tttctagttc
      421 ttgaagttta gagaatgaac agtaagcact tgaacaaagt gtattggttt cctctgaaca
      481 cgattggctt atatacccgt atcctgcagc cgtttagaat gttct
//
