LOCUS       sequence-75809-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination plasmid (Gateway cloning) for cumate-inducible 
            expression of myc-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQMD
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(83..207)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             249..314
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(386..403)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(386..402)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(395..417)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(611..630)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     730..752
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(790..808)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
       61 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
      121 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
      181 tcgttcagct ttcttgtaca aagtggtgat atcgctagct agaggtaccg agctccaatt
      241 gggatccgga ctacaaggac cacgacggcg actataagga ccacgacatc gactacaagg
      301 atgacgacga caagtgagga tccggtgaag tgacccgcaa aaaaaaaggc caggggatcg
      361 ctcccccggc cttttttttg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      421 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      481 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      541 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
      601 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
      661 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
      721 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
      781 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      841 cttagcaccc tttctcggtc cttcaacgtt cctgacaacg agcctccttt tcgccaatcc
      901 atcgacaatc accgcgagtc cctgctcgaa cgctgcgtcc ggaccgg
//
