LOCUS       sequence-75812-e         593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cumate-inducible expression of 3xFLAG-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQF
FEATURES             Location/Qualifiers
     source          1..593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..137)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(192..257)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    282..309
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    352..379
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
ORIGIN
        1 gaattcaaaa aaaaggccgg gggagcgatc ccctggcctt tttttttgcg ggtcacttca
       61 ccggatcctc acttgtcgtc gtcatccttg tagtcgatgt cgtggtcctt atagtcgccg
      121 tcgtggtcct tgtagtccgg atcccaattg gagctcggta cctcgagatc ttctagagtc
      181 atattaataa gcttgtcgtc gtcatccttg tagtcgatgt cgtggtcctt atagtcgccg
      241 tcgtggtcct tgtagtccat gcggaagctt cctctactag ttacaaacag accagattgt
      301 ctgtttgtta accggcgcag ttagcggctt cgcggcgatc cgtcaaccat gtacaaacag
      361 accagattgt ctgtttgttc cggtacattg acatatgtat ccgctcatga tacaataacc
      421 ctgatgctag cagggagaga ccccgaatgg tcatcatgtc gccgaagcgc cgcacccagg
      481 ccgagcgcgc gatggaaacc cagggcaagc tgatcgccgc cgccctgggc gtcctgcgcg
      541 agaagggcta tgccggcttc cgcatcgccg acgtcccggg cgccgccggc gtc
//
