LOCUS       sequence-75814-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cumate-inducible expression of myc-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQM
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..137)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(195..224)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    249..276
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    319..346
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
ORIGIN
        1 gaattcaaaa aaaaggccgg gggagcgatc ccctggcctt tttttttgcg ggtcacttca
       61 ccggatcctc acttgtcgtc gtcatccttg tagtcgatgt cgtggtcctt atagtcgccg
      121 tcgtggtcct tgtagtccgg atcccaattg gagctcggta cctcgagatc ttctagagtc
      181 atattaatcg agcccaggtc ctcttccgag atcagcttct gctccatgcg gaagcttcct
      241 ctactagtta caaacagacc agattgtctg tttgttaacc ggcgcagtta gcggcttcgc
      301 ggcgatccgt caaccatgta caaacagacc agattgtctg tttgttccgg tacattgaca
      361 tatgtatccg ctcatgatac aataaccctg atgctagcag ggagagaccc cgaatggtca
      421 tcatgtcgcc gaagcgccgc acccaggccg agcgcgcgat ggaaacccag ggcaagctga
      481 tcgccgccgc cctgggcgtc ctgcgcgaga agggctatgc cggcttccgc atcgccgacg
      541 tcccgggcgc cgccggcgtc agccgcggcg cccagtcgca ccacttcccg accaagctgg
      601 aactgctgct ggccaccttc gagtggctgt acgagcagat caccgagcgc agccgcgccc
      661 gcctggccaa gctgaagccc gaggacgacg tcatccagca gatgctggac gacgccgccg
      721 agttcttcct ggacgacgac ttctcgatct cgctggacct gatcgtggcc gccgaccgcg
      781 acccggcgct gcgcgagggc atccagcgca ccgtcgagcg caaccgcttc gtcgtcgagg
      841 atatgtggct gggcgtgctg gtgtcgcgcg gcctgagccg cgacgacgcg gaggacatcc
      901 tgtggctgat cttcaactcg gtccgcggcc tggcggtccg ctcgctgtgg cagaaggaca
      961 aggagcgctt cgagcgcgtc cgcaactcga ccc
//
