LOCUS       sequence-75817-e        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses an attenuated T7 RNAP under an isopropyl 
            beta-d-1-thiogalactopyranoside (IPTG) inducible control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pN565
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(55..60,61..76,77..83)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    89..108
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca
       61 attaatcatc ggctcgtata atgtgtggaa ttgtgagcgc tcacaattta ctagatgcag
      121 ttttattctc tcgccagcac tgtaataggc actaatatcc aaaccagtag ctcaattgga
      181 gtcgtctatg tgttgtttat caagcctgcg gatctccgcg aaattgtgac ttttccgcta
      241 tttagcgatc ttgttcagtg tggctttcct tcaccggcag cagattacgt tgaacagcgc
      301 atcgatctgg gtggcaacac gattaacatc gctaagaacg acttctctga catcgaactg
      361 gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag
      421 ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag
      481 cgtcaactta aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc
      541 ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc
      601 ggcaagcgcc cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac
      661 atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct
      721 gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac
      781 cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac
      841 gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc
      901 ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc
      961 atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta
     1021 gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc
     1081 cgtgcaggtg
//
