LOCUS       sequence-75818-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses an attenuated T7 RNAP under an isopropyl 
            beta-d-1-thiogalactopyranoside (IPTG) inducible control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pN565
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
       61 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
      121 ccgttaccac cgctcgaggc gctgtgcgct atggcgatct ggaagtgtcg ggacgagggg
      181 cgtcccggcg gtaaattcga cgtgcctgcg gcgcgtcgaa caaggggcgt gcccggtgtc
      241 agttcgtggc atttttcgag gcgcgacgcc atttccaagg ctcctgagca ttcgggtctg
      301 accaaaggcc gagccgttgg cggcgggcct ctttttcata ctcgtacatc tgcgcgtcgg
      361 ttggtttgcc gtaataacgg taagcccagg ccatgccttc ttgaaccatg atcgcattga
      421 tgttggtgag ttgtgtttgg ccgccggggt attgcaacgg cgcgtaaacg accccaagag
      481 tgcggccata ccgatcaacc tctttttcgg tcacttgaac ctcttggcga aaggtcaagt
      541 cggcgagccg ttggcgagca cgggagccga aggcttggcc gctttccggt gcgtcaatat
      601 cggccaatct cacgcggatg gtctgacggt tcaccaaaac gtcgatagtg tcaccgtcaa
      661 ggattcggac gacttcaccc cggaagtcgg cccaagcggg cacactgacg attaggacga
      721 cagcggccgc gaccgcgcga agggcggcaa gggcgctttt cattgtttgc ctcctgtttt
      781 caagacggct gtga
//
