LOCUS       sequence-75867-e         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian N. meningitidis Cas9 expression, human optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-NMcas
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      155..164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccgagctcgg
      121 atccactagt ccagtgtggt ggaattctgc agatgccacc atggccgcct tcaagcccaa
      181 ccccatcaac tacatcctgg gcctggacat cggcatcgcc agcgtgggct gggccatggt
      241 ggagatcgac gaggacgaga accccatctg cctgatcgac ctgggtgtgc gcgtgttcga
      301 gcgcgctgag gtgcccaaga ctggtgacag tctggctatg gctcgccggc ttgctcgctc
      361 tgttcggcgc cttactcgcc ggcgcgctca ccgccttctg cgcgctcgcc gcctgctgaa
      421 gcgcgagggt gtgctgcagg ctgccgactt cgacgagaac ggcctgatca agagcctgcc
      481 caacactcct tggcagctgc gcgctgccgc tctggaccgc aagctgactc ctctggagtg
      541 gagcgccgtg ctgctgcacc tgatcaagca ccgcggctac ctgagccagc gcaagaacga
      601 gggcgagacc gccgacaagg agctgggt
//
