LOCUS       sequence-75868-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 1, small linker insert, no 5' 
            ATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_-1_Linker
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          181..753
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(850..869)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     904..923
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaggtctcag tatccggatc cggaggcgga agcggaggag gtaccggctt
      121 gagaccctca tcctcccggg taactaagta aggatctcga ccgatcctga gaacttcagg
      181 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat
      241 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
      301 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
      361 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga
      421 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt
      481 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt
      541 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt
      601 cttattggta gaaacaacta caccctggtc atcatcctgc ctttctcttt atggttacaa
      661 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct
      721 aaccatgttc atgccttctt ctctttccta cagctcctgg gcaacgtgct ggttgttgtg
      781 ctgtctcatc attttggcaa agaattaatt cctcgacgga tcgtagatcc agacatgata
      841 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      901 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgc
//
