LOCUS       sequence-75869-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 2, small linker insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_2_Linker
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          169..741
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    814..948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(838..857)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     892..911
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaggtctcag gctccggatc cggcggcggt accaccttga gaccctcatc
      121 ctcccgggta actaagtaag gatctcgacc gatcctgaga acttcagggt gagtttgggg
      181 acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa
      241 gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga
      301 taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt
      361 tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc
      421 acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat
      481 cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga
      541 taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga
      601 aacaactaca ccctggtcat catcctgcct ttctctttat ggttacaatg atatacactg
      661 tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat
      721 gccttcttct ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat
      781 tttggcaaag aattaattcc tcgacggatc gtagatccag acatgataag atacattgat
      841 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      901 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      961 tgcattcatt ttatgtttca gg
//
