LOCUS       sequence-75870-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 3, small linker insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_3_Linker
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        29..47
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     29..46
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          144..716
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 tctgcctggg gacgtcggag caagcttgat ttaggtgaca ctatagaggt ctcaacctct
       61 ggatccggcg gcggtaccac tatgagaccc tcatcctccc gggtaactaa gtaaggatct
      121 cgaccgatcc tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg
      181 ctattgtaaa attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg
      241 gaagatgtcc cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac
      301 tctgttgaca accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa
      361 actttagctt gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta
      421 agtactttct ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag
      481 cacagtttta gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa
      541 attctggctg gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc
      601 tgcctttctc tttatggtta caatgatata cactgtttga gatgaggata aaatactctg
      661 agtccaaacc gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc
      721 tgggcaacgt gctggttgtt gtgctgtctc atcattttgg caaagaatta attcctcgac
//
