LOCUS       sequence-75875-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses split fluorescence protein to measure caspase-2 
            dimerization in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Bouchier-Hayes L, Oberst A, McStay GP, Connell S, Tait SW, Dillon 
            CP, Flanagan JM, Beere HM, Green DR
  TITLE     Characterization of cytoplasmic caspase-2 activation by induced 
            proximity.
  JOURNAL   Mol Cell. 2009 Sep 24;35(6):830-40. doi: 
            10.1016/j.molcel.2009.07.023.
  PUBMED    19782032
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.07"; journalName: "Mol Cell"; date: 
            "2009-09-24- 24"; volume: "35"; issue: "6"; pages: "830-40"
COMMENT     Sequence Label: pBiFC-HA-hCaspase 2 CARD-VC155
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          143..239
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             303..329
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc ggactctaga ggatccggta ctagaggaac
      121 tgaaaaacca gaaagttaac tggtaagttt agtctttttg tcttttattt caggtcccgg
      181 atccggtggt ggtgcaaatc aaagaactgc tcctcagtgg atgttgcctt tacttctagg
      241 cctgtacgga agtgttactt ctgctctaaa agctgcggaa ttgtacccgc gggcccacca
      301 tgtacccata cgatgttcca gattacgctc ttatggccat ggaggcccga attcggtcga
      361 ccatggccgc tgacagggga cgcaggatat tgggagtgtg tggcatgcat cctcatcatc
      421 aggaaactct aaaaaagaac cgagtggtgc tagccaaaca gctgttgttg agcgaattgt
      481 tagaacatct tctggagaag gacatcatca ccttggaaat gagggagctc atccaggcca
      541 aagtgggcag tttcagccag aatgtggaac tcctcaactt gctgcctaag aggggtcccc
      601 aagcttttga tgccttctgt gaagcactga gggagaccaa gcaaggccac ctggaggata
      661 tgttgctcac caccctttct gggcttcagc atgtactccc accgttgagc tgtgactacg
      721 acttgagtac gtcgaccgag atctctcgag gtacccgtcc ggcgtgcaaa atcccgaacg
      781 acctgaaaca gaaagtcatg aaccacgaca agcagaagaa cggcatcaag gccaacttca
      841 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
      901 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctga
//
