LOCUS       sequence-75876-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 5, small linker insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_5_Linker
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..46
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     28..45
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          143..715
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 ctgcctgggg acgtcggagc aagcttgatt taggtgacac tatagaggtc tcactgctgg
       61 gatccggcgg cggtacctca gtgagaccct catcctcccg ggtaactaag taaggatctc
      121 gaccgatcct gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc
      181 tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg
      241 aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact
      301 ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa
      361 ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa
      421 gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc
      481 acagttttag agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa
      541 ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacaccctgg tcatcatcct
      601 gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa aatactctga
      661 gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctctttcc tacagctcct
      721 gggcaacg
//
