LOCUS       sequence-75881-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 2, small linker insert, final 
            entry vector for the assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_2'_Linker
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..46
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     28..45
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctgcctgggg acgtcggagc aagcttgatt taggtgacac tatagaggtc tcaggctccg
       61 gatccggagg cggaagcgga ggaggtacct aagtgagacc ctcatcctcc cgggtaacta
      121 agtaaggatc tcgaccgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
      181 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
      241 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
      301 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
      361 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
      421 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
      481 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
      541 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacaccct
      601 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
      661 aaaatactct gagtccaaa
//
