LOCUS       sequence-75884-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses split fluorescence protein to measure caspase-2 
            dimerization in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Bouchier-Hayes L, Oberst A, McStay GP, Connell S, Tait SW, Dillon 
            CP, Flanagan JM, Beere HM, Green DR
  TITLE     Characterization of cytoplasmic caspase-2 activation by induced 
            proximity.
  JOURNAL   Mol Cell. 2009 Sep 24;35(6):830-40. doi: 
            10.1016/j.molcel.2009.07.023.
  PUBMED    19782032
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.07"; journalName: "Mol Cell"; date: 
            "2009-09-24- 24"; volume: "35"; issue: "6"; pages: "830-40"
COMMENT     Sequence Label: pBIFC-flag-hCaspase 2 prodomain-VN173
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..52
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      527..536
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(578..599)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agtgaaccgt cagaattgat ctaccatgga ctacaaagac gatgacgaca agcttgcggc
       61 cgcgaattct atggccgctg acaggggacg caggatattg ggagtgtgtg gcatgcatcc
      121 tcatcatcag gaaactctaa aaaagaaccg agtggtgcta gccaaacagc tgttgttgag
      181 cgaattgtta gaacatcttc tggagaagga catcatcacc ttggaaatga gggagctcat
      241 ccaggccaaa gtgggcagtt tcagccagaa tgtggaactc ctcaacttgc tgcctaagag
      301 gggtccccaa gcttttgatg ccttctgtga agcactgagg gagaccaagc aaggccacct
      361 ggaggatatg ttgctcacca ccctttctgg gcttcagcat gtactcccac cgttgagctg
      421 tgactacgac ttgagtctcc cttttccggt gtgtgagtcc tgtccccttt acaagaagct
      481 ccgcctgtcg acagatactg tggaacactc ctctagaaga tccatcgcca ccatggtgag
      541 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      601 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      661 gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      721 caccctgggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
      781 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      841 cgacgg
//
