LOCUS       sequence-75888-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 8, small linker insert, final 
            entry vector for the assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_8'_Linker
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     53..70
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          168..740
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    813..947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(837..856)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     891..910
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 catgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
       61 gacactatag aggtctcagc cggcggatcc ggcggcggta cctaagtgag accctcatcc
      121 tcccgggtaa ctaagtaagg atctcgaccg atcctgagaa cttcagggtg agtttgggga
      181 cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag
      241 ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat
      301 aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt
      361 cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca
      421 cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc
      481 agggtatatt atattgtact tcagcacagt tttagagaac aattgttata attaaatgat
      541 aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa
      601 acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt
      661 ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg
      721 ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt
      781 ttggcaaaga attaattcct cgacggatcg tagatccaga catgataaga tacattgatg
      841 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      901 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      961 gcattcattt tatgtttc
//
