LOCUS       sequence-75894-e        5907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  purification of Dicer1 from mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Betancur JG, Tomari Y
  TITLE     Dicer is dispensable for asymmetric RISC loading in mammals.
  JOURNAL   RNA. 2012 Jan;18(1):24-30. doi: 10.1261/rna.029785.111. Epub 2011 
            Nov 21.
  PUBMED    22106413
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.029785.111"; journalName: "RNA"; date: "2012-01"; 
            volume: "18"; issue: "1"; pages: "24-30"
COMMENT     Sequence Label: pCAGEN-SBP-DICER1
FEATURES             Location/Qualifiers
     source          1..5907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..123
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    139..163
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agaattccca tggacgagaa gaccaccggc tggcggggcg gccacgtggt ggagggcctg
       61 gccggcgagc tggagcagct gcgggccagg ctggagcacc accctcaggg ccagcgggag
      121 ccctccggcc accggtatac aagtttgtac aaaaaagcag gctccgcggc catggcaggc
      181 ctgcagctca tgacccctgc ttcctcacca atgggtcctt tctttggact gccatggcaa
      241 caagaagcaa ttcatgataa catttatacg ccaagaaaat accaggttga gctgctggaa
      301 gcagctctgg accataacac aattgtgtgc ttgaacactg gctcagggaa gacgttcatc
      361 gcggtcctgc tcaccaaaga gctggcccat cagatcaggg gcgacctcaa cccgcatgca
      421 aaaaggaccg tgttcctcgt caactctgca aaccaggttg ctcaacaagt gtcagccgtc
      481 agaactcact cggatctcaa ggttggagag tactcagacc tagaagtaaa tgcatcttgg
      541 acaaaagaga gatggagcca agagtttact aagcaccagg ttctcattat gacttgctat
      601 gtcgccttga ctgttttgaa aaatggttac ttatcactgc cagacattaa ccttttggtg
      661 tttgatgagt gtcatcttgc gattctagac cacccctatc gtgaaattat gaagctctgt
      721 gagagttgtc catcatgccc tcgaattttg ggactaactg cttccatttt aaatgggaaa
      781 tgtgatccag aggaattgga agagaagatt cagaaactgg agcgaattct caggagtgac
      841 gctgagacgg cgactgactt ggtggtcctg gacaggtaca cttctcagcc ttgtgagatt
      901 gtggtagact gtggaccgtt tactgacaga agtgggctgt atgagagatt gctgatggag
      961 ttagaagcag cacttgattt tatcaatgat tgtaatgtag ctgtacattc aaaggaaaga
     1021 gattctactt tgatttccaa acaaatcctc tcagactgcc gtgcagtgtt ggttgttctg
     1081 ggaccctggt gtgcagataa agtagctggg atgatggtaa gagaacttca gaagtacatc
     1141 aaacatgagc aagaggagct acacaggaag ttcctattgt ttacagacac cttgttaagg
     1201 aaaatacacg ccctgtgtga agagtacttc tcacctgcct cacttgacct gaagtatgta
     1261 actcccaaag tcatgaaact gctcgagatc ctgcgcaaat acaagcccta tgagcgacag
     1321 cagtttgaaa gtgttgagtg gtacaataac aggaatcagg ataattacgt gtcctggagt
     1381 gattccgagg atgacgatga tgacgaagaa attgaagaaa aagaaaagcc agagacaaat
     1441 tttccttctc cgtttaccaa tattttatgt gggattattt ttgtggaaag aagatacaca
     1501 gcagttgtcc taaacagatt gataaaggaa gctggcaaac aagatccaga gctggcttat
     1561 atcagcagca actttataac tggacacggc attggaaaga accagcctcg cagcaaacag
     1621 atggaggcgg agttcagaaa gcaggaggag gtacttagga aatttcgagc tcatgagacc
     1681 aacctgctca ttgcaacaag tgttgtagaa gagggtgtgg atattcccaa gtgcaatttg
     1741 gtggttcgtt ttgatttgcc tacagagtat cgatcatatg tccagtctaa aggacgggca
     1801 cgagcgccga tctctaatta cgtaatgtta gcagatacag acaagataaa gagctttgaa
     1861 gaagacctca agacctacaa agctattgaa aagatcttaa ggaacaagtg ttcgaagtca
     1921 gcggatggtg ctgaggcgga cgtccatgct ggtgtagatg atgaggatac tttcccgcca
     1981 tatgtgctga ggcctgatga tgggggtcct cgagtcacaa tcaacactgc cattggacac
     2041 atcaacagat attgtgctcg attaccaagt gatccgttta cgcatctagc tcccaaatgt
     2101 agaacccgag aattgcctga tggtacattt tattcaactc tttatcttcc gattaactca
     2161 cctcttcgag cctccattgt tggtccaccg atggacagtg tacggttggc tgaaagagtg
     2221 gtagctctca tttgctgtga aaaactgcac aaaattggtg aactggatga acacttgatg
     2281 ccagttggga aagagactgt taaatatgaa gaagagctgg acttacatga cgaggaggag
     2341 accagtgttc ccgggagacc agggtccaca aaacgacggc agtgctaccc caaagcaatt
     2401 ccagagtgct tgagggagag ttaccccaaa cccgaccagc cttgttacct gtatgtgata
     2461 ggaatggtct taactacccc cctccctgat gaactcaact tcaggcggcg gaagctctac
     2521 ccacctgagg acaccacaag atgctttggg atcctgacag ccaaacccat acctcagatt
     2581 cctcactttc ctgtgtatac acgctctgga gaggtcacca tatccatcga gctgaagaag
     2641 tctggtttca cattgtctca acaaatgctc gagctgatta caagattgca ccagtacata
     2701 ttctcacata ttctccggct tgagaagcct gccctggagt ttaaacctac aggcgcggag
     2761 tcagcctact gtgttctacc tcttaatgtt gttaatgact ccggcacttt ggacattgac
     2821 tttaaattca tggaggatat tgagaagtcg gaagctcgga taggcattcc tagcaccaag
     2881 tattcaaagg aaacaccgtt tgtctttaag ttagaagatt accaagatgc agtgatcatc
     2941 ccaagatacc gcaattttga tcagcctcat cgattttatg tagctgatgt atatacggat
     3001 cttaccccac tcagtaaatt tccttcccct gaatgtgaaa cttttgcaga atattataaa
     3061 acaaagtata accttgacct gaccaatctc aaccagccac tgctggatgt tgaccacgca
     3121 tcttccagac tgaacctttt gacacctcgg catttgaatc agaaggggaa agcacttcct
     3181 ctaagcagtg ctgagaagag gaaggccaaa tgggaaagtc tgcagaacaa acagatcctg
     3241 gttccggaac tctgtgctat acatccaatc ccagcatcac tgtggagaaa agcggtttgt
     3301 ctccccagca tactgtatcg ccttcactgc cttctgactg cagaggaact aagagctcag
     3361 acagccagcg atgctggtgt gggtgtcaga tcacttcccg tggattttgg ataccctaac
     3421 ttagacttcg ggtggaaaaa atctattgat agcaagtctt tcatctcaag ctgtaactcc
     3481 tcgttggctg agagtgataa ttactgtaag cacagcacaa ctgtagtccc tgaacatgct
     3541 gcacatcaag gtgctactag accctcctta gaaaaccatg accaaatgtc tgtgaactgc
     3601 aaaaggttgc ccgctgagtc acctgctaag ctccaaagtg aggtttcaac ggatcttaca
     3661 gcaattaatg gtctttctta caataaaaac cttgccaatg gcagttatga tttagttaac
     3721 agagactttt gccaaggaaa tcagctgaat tacttcaagc aggaaatacc tgtacaacca
     3781 actacctcat atcccattca gaatttgtac aattatgaga accagcccaa gcccagcaat
     3841 gaatgtcctc tactgagtaa tacgtacctt gatgggaacg ctaacacatc tacctcagat
     3901 ggaagccccg cggtgtccac gatgcctgcc atgatgaatg ctgttaaagc actcaaggac
     3961 aggatggact ctgagcagag cccttctgtt gggtactcct caaggactct tggccccaat
     4021 ccgggactga ttctccaggc tttgacactg tcaaatgcca gtgatgggtt taacctggag
     4081 cggcttgaga tgcttggcga ctccttttta aagcatgcta tcaccacata tctgttttgc
     4141 acgtaccctg atgctcatga aggccgcctt tcatatatga gaagcaaaaa ggtcagcaac
     4201 tgtaacctgt atcgccttgg caagaagaag ggactgccca gccgcatggt ggtgtcgata
     4261 ttcgatcctc ctgtgaattg gcttcctcct ggttatgtgg taaaccaaga caaaagtaac
     4321 tcagagaagt gggaaaagga tgaaatgaca aaagactgct tgctggctaa tggcaaactg
     4381 ggtgaggcct gtgaggagga ggaggacctg acgtggaggg cccccaagga ggaggccgag
     4441 gatgaggacg acttcctgga gtatgaccag gagcatatcc agtttataga cagcatgctc
     4501 atgggctcgg gcgccttcgt gaggaaaatc tctctgtcgc ccttctcagc ctccgactct
     4561 gcatacgaat ggaagatgcc caagaaggcc tccttgggca gtatgccgtt tgcctcgggc
     4621 cttgaggatt ttgattacag ctcttgggat gcgatgtgct atctggatcc tagcaaagcc
     4681 gttgaagagg atgactttgt ggtaggtttc tggaatccat cagaagagaa ctgtggcgtc
     4741 gacacaggga agcagtccat ttcttacgac ttgcacacgg agcagtgcat tgctgacaag
     4801 agcatagcgg actgtgtgga ggccctgctg ggctgctact taaccagctg cggcgagagg
     4861 gctgctcagc tcttcctctg ttcgctgggg ctgaaggtgc tcccagtaat caaaaggacc
     4921 agccgggaga aggccttgga ccctgctcag gagaacggaa gcagccaaca aaagagcctt
     4981 tcagggagct gtgcctctcc agttggccca cgctcctctg cagggaaaga cttggagtac
     5041 ggctgcctga agatcccacc gaggtgcatg tttgaccatc cagacgcaga gaaaaccctc
     5101 aaccacctga tatctgggtt tgaaactttt gagaagaaaa ttaactatag attcaagaat
     5161 aaggcttatc ttctgcaggc ttttacacac gcctcctacc actacaacac tatcactgat
     5221 tgttaccagc gcttagaatt cctgggagat gcgattttgg actacctcat aaccaagcac
     5281 ctttatgaag acccacggca gcattctcca ggggtcttga ctgacttgcg ctctgccctt
     5341 gtcaataaca ccatctttgc atcattggct gtgaagtatg actaccacaa gtactttaaa
     5401 gctgtctctc ctgagctctt tcatgttatt gatgactttg tgaagtttca gcttgagaag
     5461 aacgaaatgc aaggaatgga ctctgagctt aggagatccg aggaggatga ggagaaagaa
     5521 gaggatatcg aagtccccaa ggccatgggg gacatttttg agtctcttgc tggtgccatt
     5581 tatatggaca gtgggatgtc tctggaggtg gtctggcagg tgtactatcc gatgatgcag
     5641 cctctaatag aaaagttttc tgcaaatgtg cctcgttctc cagtgagaga attgctcgag
     5701 atggaaccag aaactgccga gtttagccca gcggagagaa cttatgatgg aaaggtcaga
     5761 gtcaccgtgg aagtggtggg aaaggggaaa tttaaaggtg ttggccggag ctacaggatc
     5821 gccaagtctg ctgcagcacg aagggccctc cgcagcctca aagctaacca gcctcaggtt
     5881 cctaacagct gaaattcctc gaggata
//
