LOCUS       sequence-75906-e        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses GAL4BD-Rab6BQ72LdeltaCSC in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Cortes C, Rzomp KA, Tvinnereim A, Scidmore MA, Wizel B
  TITLE     Chlamydia pneumoniae inclusion membrane protein Cpn0585 interacts 
            with multiple Rab GTPases.
  JOURNAL   Infect Immun. 2007 Dec;75(12):5586-96. Epub 2007 Oct 1.
  PUBMED    17908815
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2007-12"; volume: "75(12)"; pages: "5586-96. Epub 
            2007 Oct 1"
COMMENT     Sequence Label: GBK-Rab6BQ72LdeltaCSC
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             52..81
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      747..794
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      821..1008
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(876..900)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1032..1048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1032..1048)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1045..1067)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1056..1072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tcgccggaat ttgtaatacg actcactata gggcgagccg ccatcatgga ggagcagaag
       61 ctgatctcag aggaggacct gcatatggcc atggaggccg aattcatgtc cgcaggggga
      121 gattttggga atccactgag aaaattcaag ttggtgttct tgggggagca gagcgtcggg
      181 aagacgtctc tgattacgag gttcatgtac gacagcttcg acaacacata ccaggcaacc
      241 attgggattg acttcttgtc aaaaaccatg tacttggagg accgcacggt gcgactgcag
      301 ctctgggaca cagctggtct ggagaggttc cgcagcctga tccccagcta catccgggac
      361 tccacggtgg ctgtggtggt gtacgacatc acaaatctca actccttcca acagacctct
      421 aagtggatcg acgacgtcag gacagagagg ggcagtgatg ttatcatcat gctggtgggc
      481 aacaagacgg acctggctga taagaggcag ataaccatcg aggaggggga gcagcgcgcc
      541 aaagaactga gcgtcatgtt cattgagacc agtgcgaaga ctggctacaa cgtgaagcag
      601 ctttttcgac gtgtggcgtc ggctctaccc ggaatggaga atgtccagga gaaaagcaaa
      661 gaagggatga tcgacatcaa gctggacaaa ccccaggagc ccccggccag cgagggcggc
      721 taactcgacc tgcagcggcc gcataactag cataacccct tggggcctct aaacgggtct
      781 tgaggggttt tttgcgcgct tgcagccaag ctaattccgg gcgaatttct tatgatttat
      841 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
      901 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
      961 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcaagcttg
     1021 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattcc
//
