LOCUS       sequence-75910-e        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses GAL4BD-Rab10Q68LdeltaCC in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Cortes C, Rzomp KA, Tvinnereim A, Scidmore MA, Wizel B
  TITLE     Chlamydia pneumoniae inclusion membrane protein Cpn0585 interacts 
            with multiple Rab GTPases.
  JOURNAL   Infect Immun. 2007 Dec;75(12):5586-96. Epub 2007 Oct 1.
  PUBMED    17908815
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2007-12"; volume: "75(12)"; pages: "5586-96. Epub 
            2007 Oct 1"
COMMENT     Sequence Label: GBK-Rab10Q68LdeltaCC
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             52..81
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      726..773
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      800..987
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(855..879)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1011..1027)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1011..1027)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1024..1046)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1035..1051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tcgccggaat ttgtaatacg actcactata gggcgagccg ccatcatgga ggagcagaag
       61 ctgatctcag aggaggacct gcatatggcc atggaggccg aattcatggc gaagaaaacg
      121 tacgacctgc ttttcaagct gctcctgatc ggggattccg gagtggggaa gacctgcgtc
      181 ctttttcgtt tttcggatga tgccttcaat actaccttta tttccaccat aggaatagac
      241 ttcaagatca aaacagttga attacaagga aagaagatca agctacagat atgggataca
      301 gcaggcctgg agcgatttca caccatcaca acctcctact acagaggcgc aatgggtatc
      361 atgctagtat atgacatcac caatggtaaa agttttgaaa acatcagcaa atggcttaga
      421 aacatagatg agcatgccaa tgaagatgtg gaaagaatgt tactaggaaa caagtgtgat
      481 atggacgaca aaagagttgt acctaaagga aaaggagaac agattgcaag ggagcatggt
      541 attaggtttt ttgagactag tgcaaaagca aatataaaca tcgaaaaggc gttcctcacg
      601 ttagctgaag atatccttcg aaagacccct gtaaaagagc ccaacagtga aaatgtagat
      661 atcagcagtg gaggaggcgt gacaggctgg aagagcaaat gactcgacct gcagcggccg
      721 cataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgcgcgctt
      781 gcagccaagc taattccggg cgaatttctt atgatttatg atttttatta ttaaataagt
      841 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
      901 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt
      961 cgctcttatt gaccacacct ctaccggcat gcaagcttgg cgtaatcatg gtcatagctg
     1021 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaa
//
