LOCUS       sequence-75921-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector: Position 1, FRT site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_-1_FRT_+1_OA-
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        28..46
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     28..45
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(65..98)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ctgcctgggg acgtcggagc aagcttgatt taggtgacac tatagaggtc tcagtatccg
       61 gatcgaagtt cctatacttt ctagagaata ggaacttcgt accggcttga gaccctcatc
      121 ctcccgggta actaagtaag gatctcgacc gatcctgaga acttcagggt gagtttgggg
      181 acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa
      241 gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga
      301 taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt
      361 tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc
      421 acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat
      481 cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga
      541 taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga
      601 aacaactaca ccctggtcat catcctgcct ttctctttat ggttacaatg atatacactg
      661 tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgt
//
