LOCUS       sequence-75922-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector: Position 3, FRT site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_3_FRT_+1_OA-
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     53..70
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(90..123)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          194..766
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 catgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
       61 gacactatag aggtctcaac ctctggatcg aagttcctat actttctaga gaataggaac
      121 ttcgtaccac tatgagaccc tcatcctccc gggtaactaa gtaaggatct cgaccgatcc
      181 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa
      241 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
      301 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
      361 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt
      421 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct
      481 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta
      541 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
      601 gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc
      661 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
      721 gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc tgggcaacgt
      781 gctggttg
//
