LOCUS       sequence-75931-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is 
            compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites
            and a T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Takasu Y, Sajwan S, Daimon T, Osanai-Futahashi M, Uchino K, Sezutsu 
            H, Tamura T, Zurovec M
  TITLE     Efficient TALEN Construction for Bombyx mori Gene Targeting.
  JOURNAL   PLoS One. 2013 Sep 18;8(9):e73458. doi: 
            10.1371/journal.pone.0073458.
  PUBMED    24058473
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-09-18"; volume: "8(9):e73458. doi"; pages: " 
            10.1371/journal.pone.0073458"
COMMENT     Sequence Label: pBlue-TAL
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..650)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggactc gagttaagat
       61 ctgaagttga tctctccgtt gttgaacttg cgacggactt cctccagagt caaggtgcca
      121 gccttaatca tttctccgcc gatgagcagt tcctccacgc tgagaacggc accgttgcag
      181 ttggtgatgt ggttcaagcg tgtgagttga gccttgtagt tacccttgaa gtgtccagaa
      241 acgaacagga acttgaactc agtaacagag gaagggtaga ccttccacca ttcgttgggg
      301 ttgatgtgct tgtttctggt ctggttttcc tcgacgtaac gttgcatctc gtcggcctgg
      361 ccgatgggca ggttgtaacc tccggagtaa gcctttgtat cgacgatcac accgtagtcg
      421 atcggggaac ccacagtgta gatggctcca tctggcttgc gcgagccacc gaggtgcttg
      481 ccacggtaac cgtagacctt catgaagaac tccatgacct tcatttccaa gattctgtct
      541 tgggtggagt tcctagcgat ctcgatgagt tcgatgtact cgtgaggcac gtacttgagc
      601 ttgtgcctca gctctgactt cttttcctcc agttcgctct taaccaactg ggatccagcc
      661 actctgtggg aagtcctctc ggggatgcga cggttggtgc gcttgatcaa ggcaggagcg
      721 tgggggagtc ccttcttgac agcgtccaga gcgggacggc cacccaggca ggccagagcc
      781 acgaggtggt cgttggagac gaccggtgga aagcgggcag tgagcgcaac gcaattaatg
//
