LOCUS       sequence-75932-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is 
            compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites
            and a T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Takasu Y, Sajwan S, Daimon T, Osanai-Futahashi M, Uchino K, Sezutsu 
            H, Tamura T, Zurovec M
  TITLE     Efficient TALEN Construction for Bombyx mori Gene Targeting.
  JOURNAL   PLoS One. 2013 Sep 18;8(9):e73458. doi: 
            10.1371/journal.pone.0073458.
  PUBMED    24058473
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-09-18"; volume: "8(9):e73458. doi"; pages: " 
            10.1371/journal.pone.0073458"
COMMENT     Sequence Label: pBlue-TAL
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(52..72)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(52..70)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(91..107)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(91..107)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(104..126)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    115..131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(139..145,146..163,164..169))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    184..205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctatagggcg aattgggtac cgggcccccc cccgagctcc agcttttgtt ccctttagtg
       61 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      121 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      181 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccaccggtc
      241 gtctccaacg accacctcgt ggctctggcc tgcctgggtg gccgtcccgc tctggacgct
      301 gtcaagaagg gactccccca cgctcctgcc ttgatcaagc gcaccaaccg tcgcatcccc
      361 gagaggactt cccacagagt ggctggatcc cagttggtta agagcgaact ggaggaaaag
      421 aagtcagagc tgaggcacaa gctcaagtac gtgcctcacg agtacatcga actcatcgag
      481 atcgctagga actccaccca agacagaatc ttggaaatga aggtcatgga gttcttcatg
      541 aaggtctacg gttaccgtgg caagcacctc ggtggctcgc gcaagccaga tggagccatc
      601 tacactgtgg gttccccgat cgactacggt gtgatcgtcg atacaaaggc ttactccgga
      661 ggttacaacc tgcccatcgg ccaggccgac gagatgcaac gttacgtcga ggaaaaccag
      721 accagaaaca agcacatcaa ccccaacgaa tggtggaagg tctacccttc ctctgttact
      781 gagttcaagt tcctgttcgt ttctggacac ttcaagggta act
//
