LOCUS       sequence-75935-e        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses GAL4BD-Rab1AQ70Ldelta CC in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Cortes C, Rzomp KA, Tvinnereim A, Scidmore MA, Wizel B
  TITLE     Chlamydia pneumoniae inclusion membrane protein Cpn0585 interacts 
            with multiple Rab GTPases.
  JOURNAL   Infect Immun. 2007 Dec;75(12):5586-96. Epub 2007 Oct 1.
  PUBMED    17908815
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2007-12"; volume: "75(12)"; pages: "5586-96. Epub 
            2007 Oct 1"
COMMENT     Sequence Label: GBK-Rab1AQ70LdeltaCC
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             52..81
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      753..800
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      827..1014
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(882..906)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1038..1054)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1038..1054)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1051..1073)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1062..1078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tcgccggaat ttgtaatacg actcactata gggcgagccg ccatcatgga ggagcagaag
       61 ctgatctcag aggaggacct gcatatggcc atggaggccg aattcccggg gatccttatg
      121 tccagcatga atcccgaata tgattattta ttcaagttac ttctgattgg cgactcaggg
      181 gttggaaagt cttgccttct tcttaggttt gcagatgata catatacaga aagctacatc
      241 agcacaattg gtgtggattt caaaataaga actatagagt tagacgggaa aacaatcaag
      301 cttcaaatat gggacacagc aggcctggaa agatttcgaa caatcacctc cagttattac
      361 agaggagccc atggcatcat agttgtgtat gatgtgacag atcaggagtc cttcaataat
      421 gttaaacagt ggctgcagga aatagatcgt tatgccagtg aaaatgtcaa caaattgttg
      481 gtagggaaca aatgtgatct gaccacaaag aaagtagtag actacacaac agcgaaggaa
      541 tttgctgatt cccttggaat tccgtttttg gaaaccagtg ctaagaatgc aacgaatgta
      601 gaacagtctt tcatgacgat ggcagctgag attaaaaagc gaatgggtcc cggagcaaca
      661 gctggtggtg ctgagaagtc caatgttaaa attcagagca ctccagtcaa gcagtcaggt
      721 ggaggttaac tcgacctgca gcggccgcat aactagcata accccttggg gcctctaaac
      781 gggtcttgag gggttttttg cgcgcttgca gccaagctaa ttccgggcga atttcttatg
      841 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
      901 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
      961 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgca
     1021 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1081 ccacacaaca tacgagccgg aagcataa
//
